?上海易色醫療科技有限公司
Shanghai Yise Medical Technology Co.,Ltd.
雜志：Anal Bioanal Chem. 2016 Jun;408(16):4267-73. doi:
10.1007/s00216-016-9525-9. Epub 2016 Apr 13.
標題：Metabolomics reveals mycoplasma contamination interferes with the metabolism of PANC-1 cells.
作者：Yu T1, Wang Y1, Zhang H1, Johnson CH2, Jiang Y1, Li X3, Wu Z3, Liu T3, Krausz KW4, Yu A5, Gonzalez FJ4, Huang M1, Bi H6.
英文摘要：Mycoplasma contamination is a common problem in cell
culture and can alter cellular functions. Since cell metabolism
is either directly or indirectly involved in every aspect of cell function,
it is important to detect changes to the cellular metabolome after mycoplasma infection.
In this study, liquid chromatography mass spectrometry (LC/MS)-based
metabolomics was used to investigate the effect of mycoplasma contamination
on the cellular metabolism of human pancreatic carcinoma cells (PANC-1).
Multivariate analysis demonstrated that mycoplasma contamination
induced significant metabolic changes in PANC-1 cells. Twenty-three metabolites
were identified and found to be involved in arginine and purine metabolism and
energy supply. This study demonstrates that mycoplasma contamination
significantly alters cellular metabolite levels, confirming the compelling need
for routine checking of cell cultures for mycoplasma contamination,
particularly when used for metabolomics studies. Graphical abstract
Metabolomics reveals mycoplasma contamination changes the metabolome
of PANC-1 cells.
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中文摘要：支原體污染是細胞培養中的一個常見問題，并可以改變細胞的功能。由于細胞代謝直接或間接參與細胞功能的各個方面，它的變化來檢測細胞代謝組支原體感染后重要。在這項研究中，液相色譜-質譜（LC / MS）為基礎的代謝組學方法研究了支原體污染對人胰腺癌細胞的細胞代謝的影響（株）。多因素分析表明，支原體污染誘導PANC-1細胞顯著的代謝變化。二零三個代謝產物被確定，并發現參與精氨酸和嘌呤代謝和能源供應。這項研究表明，支原體污染明顯改變細胞的代謝水平，確定為支原體污染的細胞培養常規檢查的迫切需要，尤其是當用于代謝組學研究。圖形抽象代謝組學揭示支原體污染PANC-1細胞代謝的變化。